Not known Details About thr77
Not known Details About thr77
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts isn't but readily available.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the absolute best effects are...
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This guide outlines additional feasible optimization of mobile and immunoassay parameters to be certain the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a spectacular periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which lead to the temporal coordination of every mitotic occasion. Cyclin E kinds a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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